2 cellstack culture chamber Search Results


ultra-low attachment 1-layer chambers  (Corning Life Sciences)
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Corning Life Sciences ultra-low attachment 1-layer chambers
Ultra Low Attachment 1 Layer Chambers, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cellstack culture chambers  (Corning Life Sciences)
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Corning Life Sciences cellstack culture chambers
Cellstack Culture Chambers, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2-chambered cell stacks  (Corning Life Sciences)
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Corning Life Sciences 2-chambered cell stacks
2 Chambered Cell Stacks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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incorporated's cellstack® culture chamber devices  (Corning Life Sciences)
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Corning Life Sciences incorporated's cellstack® culture chamber devices
Incorporated's Cellstack® Culture Chamber Devices, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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5-chamber cellstacks  (Corning Life Sciences)
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corning® cell culture ultra-low attachment cellstack® chamber  (Corning Life Sciences)
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Corning Life Sciences corning® cell culture ultra-low attachment cellstack® chamber
Comparison of the effect of Nunclon™Δ Surface, Corning ® cell-culture surface and Corning ® ultra-low attachment surface on DC phenotype and differentiation . DC were cultured with CellGenix DC media containing 2% human AB serum in T25 cm 2 flasks that had the same surface properties as one of the three commercially available cell factories - Nunclon™Δ Surface 1-tray Cell Factories, Corning ® <t>CellSTACK</t> ® culture chambers or Corning ® cell culture ultra-low attachment CellSTACK ® chamber. DC phenotype was evaluated following lysate-loading and maturation for 16 h with LPS and IFN-γ. (A) DCs cultured in Nunclon™Δ Surface flasks (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) exhibited an overall favorable immunophenotype with significantly higher DC-LAMP (** P = 0.003) and lower CD14 (* P = 0.01) when compared to DCs cultured in Corning ® cell-culture surface flasks (surrogate of Corning ® CellSTACK ® culture chambers). Data were the mean of 6 independent experiments (i.e. DCs from 6 different individuals) in research-scale cultures ± SEM. (B) DCs that were cultured in Corning ® ultra-low attachment surface (surrogate of Corning ® ultra-low attachment cell factory chambers) expressed significantly lower CD86 (** P = 0.011) and HLA-DR (*** P = 0.0004) following lysate-loading and maturation when compared to DCs cultured in Nunclon™Δ Surface (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) and treated the same way. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) in research-scale cultures ± SEM. P values were determined with unpaired Student's t test. * denoted that P values were highly significant.
Corning® Cell Culture Ultra Low Attachment Cellstack® Chamber, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2 cellstack culture chamber  (Corning Life Sciences)
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Corning Life Sciences 2 cellstack culture chamber
Comparison of the effect of Nunclon™Δ Surface, Corning ® cell-culture surface and Corning ® ultra-low attachment surface on DC phenotype and differentiation . DC were cultured with CellGenix DC media containing 2% human AB serum in T25 cm 2 flasks that had the same surface properties as one of the three commercially available cell factories - Nunclon™Δ Surface 1-tray Cell Factories, Corning ® <t>CellSTACK</t> ® culture chambers or Corning ® cell culture ultra-low attachment CellSTACK ® chamber. DC phenotype was evaluated following lysate-loading and maturation for 16 h with LPS and IFN-γ. (A) DCs cultured in Nunclon™Δ Surface flasks (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) exhibited an overall favorable immunophenotype with significantly higher DC-LAMP (** P = 0.003) and lower CD14 (* P = 0.01) when compared to DCs cultured in Corning ® cell-culture surface flasks (surrogate of Corning ® CellSTACK ® culture chambers). Data were the mean of 6 independent experiments (i.e. DCs from 6 different individuals) in research-scale cultures ± SEM. (B) DCs that were cultured in Corning ® ultra-low attachment surface (surrogate of Corning ® ultra-low attachment cell factory chambers) expressed significantly lower CD86 (** P = 0.011) and HLA-DR (*** P = 0.0004) following lysate-loading and maturation when compared to DCs cultured in Nunclon™Δ Surface (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) and treated the same way. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) in research-scale cultures ± SEM. P values were determined with unpaired Student's t test. * denoted that P values were highly significant.
2 Cellstack Culture Chamber, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cellstack culture chambers corning catalog #cls3271  (Corning Life Sciences)
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Corning Life Sciences cellstack culture chambers corning catalog #cls3271
Comparison of the effect of Nunclon™Δ Surface, Corning ® cell-culture surface and Corning ® ultra-low attachment surface on DC phenotype and differentiation . DC were cultured with CellGenix DC media containing 2% human AB serum in T25 cm 2 flasks that had the same surface properties as one of the three commercially available cell factories - Nunclon™Δ Surface 1-tray Cell Factories, Corning ® <t>CellSTACK</t> ® culture chambers or Corning ® cell culture ultra-low attachment CellSTACK ® chamber. DC phenotype was evaluated following lysate-loading and maturation for 16 h with LPS and IFN-γ. (A) DCs cultured in Nunclon™Δ Surface flasks (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) exhibited an overall favorable immunophenotype with significantly higher DC-LAMP (** P = 0.003) and lower CD14 (* P = 0.01) when compared to DCs cultured in Corning ® cell-culture surface flasks (surrogate of Corning ® CellSTACK ® culture chambers). Data were the mean of 6 independent experiments (i.e. DCs from 6 different individuals) in research-scale cultures ± SEM. (B) DCs that were cultured in Corning ® ultra-low attachment surface (surrogate of Corning ® ultra-low attachment cell factory chambers) expressed significantly lower CD86 (** P = 0.011) and HLA-DR (*** P = 0.0004) following lysate-loading and maturation when compared to DCs cultured in Nunclon™Δ Surface (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) and treated the same way. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) in research-scale cultures ± SEM. P values were determined with unpaired Student's t test. * denoted that P values were highly significant.
Cellstack Culture Chambers Corning Catalog #Cls3271, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1-stack culture vessels  (Corning Life Sciences)
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Corning Life Sciences 1-stack culture vessels
Comparison of the effect of Nunclon™Δ Surface, Corning ® cell-culture surface and Corning ® ultra-low attachment surface on DC phenotype and differentiation . DC were cultured with CellGenix DC media containing 2% human AB serum in T25 cm 2 flasks that had the same surface properties as one of the three commercially available cell factories - Nunclon™Δ Surface 1-tray Cell Factories, Corning ® <t>CellSTACK</t> ® culture chambers or Corning ® cell culture ultra-low attachment CellSTACK ® chamber. DC phenotype was evaluated following lysate-loading and maturation for 16 h with LPS and IFN-γ. (A) DCs cultured in Nunclon™Δ Surface flasks (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) exhibited an overall favorable immunophenotype with significantly higher DC-LAMP (** P = 0.003) and lower CD14 (* P = 0.01) when compared to DCs cultured in Corning ® cell-culture surface flasks (surrogate of Corning ® CellSTACK ® culture chambers). Data were the mean of 6 independent experiments (i.e. DCs from 6 different individuals) in research-scale cultures ± SEM. (B) DCs that were cultured in Corning ® ultra-low attachment surface (surrogate of Corning ® ultra-low attachment cell factory chambers) expressed significantly lower CD86 (** P = 0.011) and HLA-DR (*** P = 0.0004) following lysate-loading and maturation when compared to DCs cultured in Nunclon™Δ Surface (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) and treated the same way. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) in research-scale cultures ± SEM. P values were determined with unpaired Student's t test. * denoted that P values were highly significant.
1 Stack Culture Vessels, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cellstack culture chambers  (Avantor)
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Avantor cellstack culture chambers
Comparison of the effect of Nunclon™Δ Surface, Corning ® cell-culture surface and Corning ® ultra-low attachment surface on DC phenotype and differentiation . DC were cultured with CellGenix DC media containing 2% human AB serum in T25 cm 2 flasks that had the same surface properties as one of the three commercially available cell factories - Nunclon™Δ Surface 1-tray Cell Factories, Corning ® <t>CellSTACK</t> ® culture chambers or Corning ® cell culture ultra-low attachment CellSTACK ® chamber. DC phenotype was evaluated following lysate-loading and maturation for 16 h with LPS and IFN-γ. (A) DCs cultured in Nunclon™Δ Surface flasks (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) exhibited an overall favorable immunophenotype with significantly higher DC-LAMP (** P = 0.003) and lower CD14 (* P = 0.01) when compared to DCs cultured in Corning ® cell-culture surface flasks (surrogate of Corning ® CellSTACK ® culture chambers). Data were the mean of 6 independent experiments (i.e. DCs from 6 different individuals) in research-scale cultures ± SEM. (B) DCs that were cultured in Corning ® ultra-low attachment surface (surrogate of Corning ® ultra-low attachment cell factory chambers) expressed significantly lower CD86 (** P = 0.011) and HLA-DR (*** P = 0.0004) following lysate-loading and maturation when compared to DCs cultured in Nunclon™Δ Surface (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) and treated the same way. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) in research-scale cultures ± SEM. P values were determined with unpaired Student's t test. * denoted that P values were highly significant.
Cellstack Culture Chambers, supplied by Avantor, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cellbind™ cellstack™ 2-chamber  (Corning Life Sciences)
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Corning Life Sciences cellbind™ cellstack™ 2-chamber
Comparison of the effect of Nunclon™Δ Surface, Corning ® cell-culture surface and Corning ® ultra-low attachment surface on DC phenotype and differentiation . DC were cultured with CellGenix DC media containing 2% human AB serum in T25 cm 2 flasks that had the same surface properties as one of the three commercially available cell factories - Nunclon™Δ Surface 1-tray Cell Factories, Corning ® <t>CellSTACK</t> ® culture chambers or Corning ® cell culture ultra-low attachment CellSTACK ® chamber. DC phenotype was evaluated following lysate-loading and maturation for 16 h with LPS and IFN-γ. (A) DCs cultured in Nunclon™Δ Surface flasks (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) exhibited an overall favorable immunophenotype with significantly higher DC-LAMP (** P = 0.003) and lower CD14 (* P = 0.01) when compared to DCs cultured in Corning ® cell-culture surface flasks (surrogate of Corning ® CellSTACK ® culture chambers). Data were the mean of 6 independent experiments (i.e. DCs from 6 different individuals) in research-scale cultures ± SEM. (B) DCs that were cultured in Corning ® ultra-low attachment surface (surrogate of Corning ® ultra-low attachment cell factory chambers) expressed significantly lower CD86 (** P = 0.011) and HLA-DR (*** P = 0.0004) following lysate-loading and maturation when compared to DCs cultured in Nunclon™Δ Surface (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) and treated the same way. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) in research-scale cultures ± SEM. P values were determined with unpaired Student's t test. * denoted that P values were highly significant.
Cellbind™ Cellstack™ 2 Chamber, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cf5 polystyrene cellstack culture chamber  (Corning Life Sciences)
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Corning Life Sciences cf5 polystyrene cellstack culture chamber
Comparison of the effect of Nunclon™Δ Surface, Corning ® cell-culture surface and Corning ® ultra-low attachment surface on DC phenotype and differentiation . DC were cultured with CellGenix DC media containing 2% human AB serum in T25 cm 2 flasks that had the same surface properties as one of the three commercially available cell factories - Nunclon™Δ Surface 1-tray Cell Factories, Corning ® <t>CellSTACK</t> ® culture chambers or Corning ® cell culture ultra-low attachment CellSTACK ® chamber. DC phenotype was evaluated following lysate-loading and maturation for 16 h with LPS and IFN-γ. (A) DCs cultured in Nunclon™Δ Surface flasks (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) exhibited an overall favorable immunophenotype with significantly higher DC-LAMP (** P = 0.003) and lower CD14 (* P = 0.01) when compared to DCs cultured in Corning ® cell-culture surface flasks (surrogate of Corning ® CellSTACK ® culture chambers). Data were the mean of 6 independent experiments (i.e. DCs from 6 different individuals) in research-scale cultures ± SEM. (B) DCs that were cultured in Corning ® ultra-low attachment surface (surrogate of Corning ® ultra-low attachment cell factory chambers) expressed significantly lower CD86 (** P = 0.011) and HLA-DR (*** P = 0.0004) following lysate-loading and maturation when compared to DCs cultured in Nunclon™Δ Surface (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) and treated the same way. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) in research-scale cultures ± SEM. P values were determined with unpaired Student's t test. * denoted that P values were highly significant.
Cf5 Polystyrene Cellstack Culture Chamber, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Comparison of the effect of Nunclon™Δ Surface, Corning ® cell-culture surface and Corning ® ultra-low attachment surface on DC phenotype and differentiation . DC were cultured with CellGenix DC media containing 2% human AB serum in T25 cm 2 flasks that had the same surface properties as one of the three commercially available cell factories - Nunclon™Δ Surface 1-tray Cell Factories, Corning ® CellSTACK ® culture chambers or Corning ® cell culture ultra-low attachment CellSTACK ® chamber. DC phenotype was evaluated following lysate-loading and maturation for 16 h with LPS and IFN-γ. (A) DCs cultured in Nunclon™Δ Surface flasks (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) exhibited an overall favorable immunophenotype with significantly higher DC-LAMP (** P = 0.003) and lower CD14 (* P = 0.01) when compared to DCs cultured in Corning ® cell-culture surface flasks (surrogate of Corning ® CellSTACK ® culture chambers). Data were the mean of 6 independent experiments (i.e. DCs from 6 different individuals) in research-scale cultures ± SEM. (B) DCs that were cultured in Corning ® ultra-low attachment surface (surrogate of Corning ® ultra-low attachment cell factory chambers) expressed significantly lower CD86 (** P = 0.011) and HLA-DR (*** P = 0.0004) following lysate-loading and maturation when compared to DCs cultured in Nunclon™Δ Surface (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) and treated the same way. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) in research-scale cultures ± SEM. P values were determined with unpaired Student's t test. * denoted that P values were highly significant.

Journal: Journal of Translational Medicine

Article Title: Optimizing parameters for clinical-scale production of high IL-12 secreting dendritic cells pulsed with oxidized whole tumor cell lysate

doi: 10.1186/1479-5876-9-198

Figure Lengend Snippet: Comparison of the effect of Nunclon™Δ Surface, Corning ® cell-culture surface and Corning ® ultra-low attachment surface on DC phenotype and differentiation . DC were cultured with CellGenix DC media containing 2% human AB serum in T25 cm 2 flasks that had the same surface properties as one of the three commercially available cell factories - Nunclon™Δ Surface 1-tray Cell Factories, Corning ® CellSTACK ® culture chambers or Corning ® cell culture ultra-low attachment CellSTACK ® chamber. DC phenotype was evaluated following lysate-loading and maturation for 16 h with LPS and IFN-γ. (A) DCs cultured in Nunclon™Δ Surface flasks (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) exhibited an overall favorable immunophenotype with significantly higher DC-LAMP (** P = 0.003) and lower CD14 (* P = 0.01) when compared to DCs cultured in Corning ® cell-culture surface flasks (surrogate of Corning ® CellSTACK ® culture chambers). Data were the mean of 6 independent experiments (i.e. DCs from 6 different individuals) in research-scale cultures ± SEM. (B) DCs that were cultured in Corning ® ultra-low attachment surface (surrogate of Corning ® ultra-low attachment cell factory chambers) expressed significantly lower CD86 (** P = 0.011) and HLA-DR (*** P = 0.0004) following lysate-loading and maturation when compared to DCs cultured in Nunclon™Δ Surface (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) and treated the same way. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) in research-scale cultures ± SEM. P values were determined with unpaired Student's t test. * denoted that P values were highly significant.

Article Snippet: Three commercially available cell factories were investigated- 1) NunclonTMΔ Surface 1-tray Cell Factories, 2) Corning ® CellSTACK ® culture chambers and 3) Corning ® cell culture ultra-low attachment CellSTACK ® chamber.

Techniques: Comparison, Cell Culture